Phytochemical composition of Verbascum stachydifolium Boiss & Heldr. var. stachydifolium growing in Türkiye and in vitro analysis of wound healing activity

This study aimed to investigate the phenolic content, antioxidant activity, cytotoxicity and in vitro wound healing activity of methanolic aqueous extracts Verbascum stachydifolium Boiss & Heldr. var. stachydifolium. Total flavonoid contents were measured using spectrophotometry-based methods. Quantitative analysis selected phenolics was performed by HPLC. The cytotoxic effects on L929 mouse fibroblast cells evaluated MTT assay. migration treated assessed cell scratch expressions type I collagen, FGF7, TGF-?1 VEGF qRT-PCR ELISA. HPLC-based revealed presence different compounds at varying amounts high activities detected. assay results indicated that did not exhibit any effect when used up 500 ?g/mL concentration. Fibroblast stimulated highest degree extract obtained maceration as observed 60.4% closure. molecular mechanism involves upregulation analyzed genes.